Goat anti-Mcpt4 / Myonase (mouse) Antibody

Item#:

dAP-1731

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Mcpt4 / Myonase (mouse) Antibody
Principle Name	Mcpt4 / Myonase (mouse)
Official Symbol	Mcpt4
All Name and Symbols	Mcpt4; mast cell protease 4; MMCP-4; MMCP-4A; MMCP-4B; Mcp-4; myonase
Accession Number(s)	NP_034909.2
Peptide Sequence	KLQKKAKETPSVN, is from internal region
Human Gene ID(s)
Non-Human GeneID(s)	17227 (mouse);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, IHC
Tested Species	Mouse
Peptide ELISA	antibody detection limit dilution 1 to 64000.
Western Blot	Preliminary experiments gave bands at approx. 40kDa and 15kDa in Mouse Skeletal Muscle lysates after 0.01µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the
Immunohistochemistry	Paraffin embedded Mouse Skeletal Muscle. Recommended concentration
Reference(s)	Kitaura-Inenaga K, Hara M, Higuchi K, Yamamoto K, Yamaki A, Ono K, Nakano A, Kinoshita M, Sasayama S, Matsumori A. Gene expression of cardiac mast cell chymase and tryptase in a murine model of heart failure caused by viral myocarditis. Circ. J. 2003 Oct 67 (10)