Goat anti-TREX2 Antibody |
| Principle Name | TREX2 |
| Official Symbol | TREX2 |
| All Name and Symbols | three prime repair exonuclease 2; 3'-5' exonuclease |
| Accession Number(s) | NP_542432.2 |
| Peptide Sequence | HRSSLENPEHDES, is from internal region |
| Human Gene ID(s) | 11219 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Preliminary experiments gave an approx 18kDa band in lysates of Jurkat, MOLT4 and U937 after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculate |
| Immunohistochemistry | In paraffin embedded Human Breast shows nuclear staining in the luminal epithelial duct cells. Recommended concentration, 3-6µg/ml. |
| Reference(s) | de Silva U, Choudhury S, Bailey SL, Harvey S, Perrino FW, Hollis T. The crystal structure of TREX1 explains the 3' nucleotide specificity and reveals a polyproline II helix for protein partnering. J Biol Chem. 2007 Apr;282(14) |
