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Goat anti-EWS / EWSR1 Antibody
Item#:
dAP-1718
Size Price Quantity
50.0 ug $310.00
Goat anti-EWS / EWSR1 Antibody
Principle Name EWS / EWSR1
Official Symbol EWSR1
All Name and Symbols EWSR1; EWS; Ewing sarcoma breakpoint region 1; EWS/FLI fusion protein; EWSR1/ZNF384 fusion protein; Ewings sarcoma EWS-Fli1 (type 1) oncogene; bK984G1.4 (Ewing sarcoma breakpoint region 1 protein); extraskeletal myxoid chondrosarcoma EWS/TEC/CHN fusion pr
Accession Number(s) NP_053733.2; NP_005234.1; NP_001156757.1; NP_001156758.1; NP_001156759.1
Peptide Sequence TSYDQSSYSQQNTYG, is from internal region
Human Gene ID(s) 2130
Non-Human GeneID(s) 14030 (mouse); 289752 (rat);
Important Comments This antibody is expected to recognize all five reported isoforms (NP_053733.2; NP_005234.1; NP_001156757.1; NP_001156758.1; NP_001156759.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 64000.
Western Blot Approx. 90kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 68.5kDa according to NP_053733.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Rec
Reference(s) Toretsky JA, Erkizan V, Levenson A, Abaan OD, Parvin JD, Cripe TP, Rice AM, Lee SB, Uren A. Oncoprotein EWS-FLI1 activity is enhanced by RNA helicase A. Cancer Res. 2006 Jun 1;66(11)

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