Goat anti-DNA polymerase theta Antibody

Item#:

dAP-1703

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-DNA polymerase theta Antibody
Principle Name	DNA polymerase theta
Official Symbol	POLQ
All Name and Symbols	POLQ ; polymerase (DNA directed), theta ; DKFZp781A0112; POLH; PRO0327; DNA polymerase eta; DNA polymerase theta
Accession Number(s)	NP_955452.3
Peptide Sequence	DTHQQLTCSNDES, is from internal region
Human Gene ID(s)	10721
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 1000.
Western Blot	Preliminary experiments in Human Brain (Cerebellum lysates) and in lysates of cell line HeLa gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - ha
Reference(s)	Kawamura K, Bahar R, Seimiya M, Chiyo M, Wada A, Okada S, Hatano M, Tokuhisa T, Kimura H, Watanabe S, Honda I, Sakiyama S, Tagawa M, O-Wang J. DNA polymerase theta is preferentially expressed in lymphoid tissues and upregulated in human cancers. Int J Cancer. 2004 Mar;109(1)