Goat anti-HAMP Antibody |
| Principle Name | HAMP |
| Official Symbol | HAMP |
| All Name and Symbols | HAMP; hepcidin antimicrobial peptide; HEPC; HEPCIDIN; HFE2B; LEAP-1; LEAP1; PLTR; liver-expressed antimicrobial peptide; putative liver tumor regressor |
| Accession Number(s) | NP_066998.1 |
| Peptide Sequence | TSGSVFPQQTGQLAE, is from N Terminus (near) |
| Human Gene ID(s) | 57817 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Preliminary experiments gave an approx 28kDa band in Human Liver lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 9.41k |
| Immunohistochemistry | Paraffin embedded Human Heart. Recommended concentration |
| Reference(s) | Ward DG, Roberts K, Brookes MJ, Joy H, Martin A, Ismail T, Spychal R, Iqbal T, Tselepis C. Increased hepcidin expression in colorectal carcinogenesis. World J Gastroenterol. 2008 Mar 7;14(9) |
