Goat anti-SFRP4 Antibody |
| Principle Name | SFRP4 |
| Official Symbol | SFRP4 |
| All Name and Symbols | SFRP4; secreted frizzled-related protein 4; FRP-4; FRPHE; MGC26498; secreted frizzled-related protein 4 |
| Accession Number(s) | NP_003005.2 |
| Peptide Sequence | TNSSCQCPHILPHQD, is from internal region |
| Human Gene ID(s) | 6424 |
| Non-Human GeneID(s) | 20379 (mouse); 89803 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 40kDa band observed in Human Uterus lysates (calculated MW of 39.8kDa according to NP_003005.2). Recommended concentration |
| Immunohistochemistry | In paraffin embedded Human Uterus shows granular staining in the apical cytoplasm of the endometrial gland. Recommended concentration, 2-4µg/ml. |
| Reference(s) | Linhart HG, Lin H, Yamada Y, Moran E, Steine EJ, Gokhale S, Lo G, Cantu E, Ehrich M, He T, Meissner A, Jaenisch R. Dnmt3b promotes tumorigenesis in vivo by gene-specific de novo methylation and transcriptional silencing. Genes Dev. 2007 Dec 1;21(23) |
