Goat anti-ALOX15 Antibody

Item#:

dAP-1686

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-ALOX15 Antibody
Principle Name	ALOX15
Official Symbol	ALOX15
All Name and Symbols	ALOX15; arachidonate 15-lipoxygenase; 15-LOX-1; 15-LOX2
Accession Number(s)	NP_001131.3
Peptide Sequence	HYKTDVAVKDDPE, is from internal region
Human Gene ID(s)	246
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx. 80kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 74.8kDa according to NP_001131.3). Recommended concentration
Immunohistochemistry	Paraffin embedded Human Skeletal Muscle and Kidney. Recommended concentration
Reference(s)	McDuffie M, Maybee NA, Keller SR, Stevens BK, Garmey JC, Morris MA, Kropf E, Rival C, Ma K, Carter JD, Tersey SA, Nunemaker CS, Nadler JL. Nonobese diabetic (NOD) mice congenic for a targeted deletion of 12/15-lipoxygenase are protected from autoimmune diabetes. Diabetes. 2008 Jan;57(1)