Goat anti-ITIH4 Antibody |
| Principle Name | ITIH4 |
| Official Symbol | ITIH4 |
| All Name and Symbols | ITIH4; inter-alpha (globulin) inhibitor H4 (plasma Kallikrein-sensitive glycoprotein); DKFZp686G21125; H4P; IHRP; ITIHL1; PK120; inter-alpha (globulin) inhibitor H4; plasma kallikrein-sensitive glycoprotein |
| Accession Number(s) | NP_002209.2 |
| Peptide Sequence | ERLCVDPRHRQGPVN, is from internal region |
| Human Gene ID(s) | 3700 |
| Non-Human GeneID(s) | |
| Important Comments | The immunizing peptide represents a region of the 35kDa chain. This chain is glycosylated giving rise to an apparent higher molecular weight. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Preliminary experiments gave an approx. 150kDa band in Human Liver and Lung lysates after 0.03µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated s |
| Immunohistochemistry | In paraffin embedded Human Liver shows vesicular staining in the cytoplasm of hepatocytes. Recommended concentration, 4-6µg/ml. |
| Reference(s) | Tang Y, Kitisin K, Jogunoori W, Li C, Deng CX, Mueller SC, Ressom HW, Rashid A, He AR, Mendelson JS, Jessup JM, Shetty K, Zasloff M, Mishra B, Reddy EP, Johnson L, Mishra L. Progenitor/stem cells give rise to liver cancer due to aberrant TGF-beta and IL-6 signaling. Proc Natl Acad Sci U S A. 2008 Feb 19;105(7) |
