Goat anti-MTNR1B Antibody

Item#:

dAP-1660

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-MTNR1B Antibody
Principle Name	MTNR1B
Official Symbol	MTNR1B
All Name and Symbols	MTNR1B; melatonin receptor 1B; MEL-1B-R; MT2; melatonin receptor MEL1B; melatonin receptor type 1B
Accession Number(s)	NP_005950.1
Peptide Sequence	QDASKGSHAEGLQSP, is from C Terminus
Human Gene ID(s)	4544
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Preliminary experiments gave approx .48kDa and 37kDa bands in Human Brain (Cerebellum) lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calc
Immunohistochemistry	In paraffin embedded Human Cerebellum shows membranous staining primarily in Purkinje cells. Recommended concentration, 3-6µg/ml.
Reference(s)	Rawashdeh O, de Borsetti NH, Roman G, Cahill GM. Melatonin suppresses nighttime memory formation in zebrafish. Science 2007 Nov 318 (5853)