Goat anti-GABRB3 Antibody |
| Principle Name | GABRB3 |
| Official Symbol | GABRB3 |
| All Name and Symbols | GABRB3; gamma-aminobutyric acid (GABA) A receptor, beta 3; MGC9051; GABA(A) receptor beta-3 subunit; GABA-A receptor beta-3 subunit; GABA-alpha receptor beta-2 subunit; GABAA receptor beta-3 subunit |
| Accession Number(s) | NP_000805.1; NP_068712.1 |
| Peptide Sequence | EKTAKAKNDRSK, is from internal region |
| Human Gene ID(s) | 2562 |
| Non-Human GeneID(s) | 14402 (mouse); 24922 (rat); |
| Important Comments | This antibody is expected to recognize both reported isoforms (NP_000805.1, NP_068712.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 8000. |
| Western Blot | Preliminary experiments in Human Brain (Cerebral Cortex, Frontal Cortex and Cerebellum) lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - |
| Immunohistochemistry | In paraffin embedded Human Cortex shows patchy staining near or at the neuronal cell membrane.. Recommended concentration, 2-4µg/ml. |
| Reference(s) | Tanaka M, Olsen RW, Medina MT, Schwartz E, Alonso ME, Duron RM, Castro-Ortega R, Martinez-Juarez IE, Pascual-Castroviejo I, Machado-Salas J, Silva R, Bailey JN, Bai D, Ochoa A, Jara-Prado A, Pineda G, Macdonald RL, Delgado-Escueta AV. Hyperglycosylation and reduced GABA currents of mutated GABRB3 polypeptide in remitting childhood absence epilepsy. Am. J. Hum. Genet. 2008 Jun 82 (6) |
