Goat anti-GAD1 (isoform GAD67) Antibody

Item#:

dAP-1654

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-GAD1 (isoform GAD67) Antibody
Principle Name	GAD1 (isoform GAD67)
Official Symbol	GAD1
All Name and Symbols	GAD1; glutamate decarboxylase 1 (brain, 67kDa); FLJ45882; GAD; SCP; OTTHUMP00000041055; glutamate decarboxylase 1; glutamate decarboxylase 1 (brain, 67kD); GAD67; GAD25
Accession Number(s)	NP_000808.2
Peptide Sequence	PDSPQRREKLHK, is from internal region
Human Gene ID(s)	2571
Non-Human GeneID(s)	14415 (mouse); 24379 (rat);
Important Comments	This antibody is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2.
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx 65kDa band observed in Human Brain (Cerebral Cortex) lysates (calculated MW of 66.9kDa according to NP_000808.2). Recommended concentration
Reference(s)	Huang HS, Akbarian S. GAD1 mRNA expression and DNA methylation in prefrontal cortex of subjects with schizophrenia. PLoS ONE 2007 2 (8)