Goat anti-PHOX2B Antibody

Item#:

dAP-1642

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-PHOX2B Antibody
Principle Name	PHOX2B
Official Symbol	PHOX2B
All Name and Symbols	PHOX2B; paired-like homeobox 2b; NBPhox; PMX2B; PHOX2B homeodomain protein; neuroblastoma paired-type homeobox protein; paired mesoderm homeobox 2b
Accession Number(s)	NP_003915.2
Peptide Sequence	SRDDESKEAKSTDP, is from internal region
Human Gene ID(s)	8929
Non-Human GeneID(s)	18935 (mouse);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 1000.
Western Blot	Preliminary experiments in Human Brain (Cerebellum) lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been reported with
Reference(s)	McGaughey DM, Vinton RM, Huynh J, Al-Saif A, Beer MA, McCallion AS. Metrics of sequence constraint overlook regulatory sequences in an exhaustive analysis at phox2b. Genome Res. 2008 Feb;18(2)