Goat anti-APOL1 Antibody |
| Principle Name | APOL1 |
| Official Symbol | APOL1 |
| All Name and Symbols | APOL1; apolipoprotein L, 1; APO-L; APOL; APOL-I; OTTHUMP00000028705; apolipoprotein L-I; apolipoprotein L1 |
| Accession Number(s) | NP_003652.2; NP_663318.1 |
| Peptide Sequence | NNNYKILQADQE, is from C Terminus |
| Human Gene ID(s) | 8542 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognize both reported isoforms (NP_003652.2; NP_663318.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Approx 45kDa band observed in Human Placenta lysates (calculated MW of 44kDa according to NP_003652.2 and of 45.9kDa according to NP_663318.1 ). Recommended concentration |
| Reference(s) | Pérez-Morga D, Vanhollebeke B, Paturiaux-Hanocq F, Nolan DP, Lins L, Homblé F, Vanhamme L, Tebabi P, Pays A, Poelvoorde P, Jacquet A, Brasseur R, Pays E. Apolipoprotein L-I promotes trypanosome lysis by forming pores in lysosomal membranes. Science. 2005 Jul 15;309(5733) |
