Goat anti-CHKB Antibody |
| Principle Name | CHKB |
| Official Symbol | CHKB |
| All Name and Symbols | CHKB; choline kinase beta; CHETK; CHKL; choline kinase-like; choline/ethanolamine kinase |
| Accession Number(s) | NP_005189.2 |
| Peptide Sequence | KGETLSQEEQRKLEED, is from internal region |
| Human Gene ID(s) | 1120 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognize isoform a (NP_005189.2) only. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA |
| Tested Species | |
| Peptide ELISA | antibody detection limit dilution 1 to 2000. |
| Western Blot | Preliminary experiments gave an approx 38kDa band in Human Skeletal Muscle lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size |
| Reference(s) | Aoyama C, Liao H, Ishidate K. Structure and function of choline kinase isoforms in mammalian cells. Prog Lipid Res. 2004 May;43(3) |
