Goat anti-PARP3 Antibody |
| Principle Name | PARP3 |
| Official Symbol | PARP3 |
| All Name and Symbols | PARP3; poly (ADP-ribose) polymerase family, member 3; ADPRT3; ADPRTL2; ADPRTL3; IRT1; hPARP-3; pADPRT-3; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 2; ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase)-like 3; NAD+ ADP-ribos |
| Accession Number(s) | NP_001003931.2; NP_001003935.2 |
| Peptide Sequence | YEDYNCTLNQTNIEN , is from internal region |
| Human Gene ID(s) | 10039 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognize both reported isoforms (NP_001003931.2; NP_001003935.2). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 2000. |
| Western Blot | Preliminary experiments in lysates of cell lines Daudi, HeLa and HepG2 gave no specific signal but low background (at antibody concentration up to 1µg/ml). |
| Immunohistochemistry | Paraffin embedded Human Liver and Kidney. Recommended concentration |
| Reference(s) | Rouleau M, McDonald D, Gagné P, Ouellet ME, Droit A, Hunter JM, Dutertre S, Prigent C, Hendzel MJ, Poirier GG. PARP-3 associates with polycomb group bodies and with components of the DNA damage repair machinery. J Cell Biochem. 2007 Feb 1;100(2) |
