Goat anti-SIGLEC8 Antibody

Item#:

dAP-1624

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-SIGLEC8 Antibody
Principle Name	SIGLEC8
Official Symbol	SIGLEC8
All Name and Symbols	SIGLEC8; sialic acid binding Ig-like lectin 8; MGC59785; SAF2; SIGLEC-8; SIGLEC8L
Accession Number(s)	NP_055257.2
Peptide Sequence	SYKSQLNYKTKQ, is from internal region
Human Gene ID(s)	27181
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx. 45kDa band observed in lysates of cell line MOLT4 (calculated MW of 54.0kDa according to NP_055257.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Nutku at al, Blood. 2003 J
Reference(s)	Yokoi H, Choi OH, Hubbard W, Lee HS, Canning BJ, Lee HH, Ryu SD, von Gunten S, Bickel CA, Hudson SA, Macglashan DW Jr, Bochner BS. Inhibition of FcepsilonRI-dependent mediator release and calcium flux from human mast cells by sialic acid-binding immunoglobulin-like lectin 8 engagement. J Allergy Clin Immunol. 2008 Feb;121(2)