Goat anti-HEM1 Antibody |
| Principle Name | HEM1 |
| Official Symbol | NCKAP1L |
| All Name and Symbols | NCKAP1L; NCK-associated protein 1-like; HEM1; hematopoietic protein 1; membrane-associated protein hem-1 |
| Accession Number(s) | NP_005328.2 |
| Peptide Sequence | RHTENVTKTKTPE, is from internal region |
| Human Gene ID(s) | 3071 |
| Non-Human GeneID(s) | 105855 (mouse); 315348 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA |
| Tested Species | |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Preliminary experiments gave an approx. 50kDa band in Human Tonsil and Thymus lysates after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated |
| Reference(s) | Weiner OD, Rentel MC, Ott A, Brown GE, Jedrychowski M, Yaffe MB, Gygi SP, Cantley LC, Bourne HR, Kirschner MW. Hem-1 complexes are essential for Rac activation, actin polymerization, and myosin regulation during neutrophil chemotaxis. PLoS Biol. 2006 Feb;4(2) |
