Goat anti-GRIA4 Antibody |
| Principle Name | GRIA4 |
| Official Symbol | GRIA4 |
| All Name and Symbols | GRIA4 ; glutamate receptor, ionotrophic, AMPA 4 ; GLUR4; GLUR4C; GLURD; AMPA-selective glutamate receptor 4 |
| Accession Number(s) | NP_000820.3; NP_001070711.1; NP_001070712.1 |
| Peptide Sequence | KKLDQREYPGSETP, is from internal region |
| Human Gene ID(s) | 2893 |
| Non-Human GeneID(s) | 14802 (mouse); 29629 (rat); |
| Important Comments | This antibody is expected to recognize all reported isforms (NP_000820.3; NP_001070711.1; NP_001070712.1). Reported variants NP_001070712.1 and NP_001106283.1 represent identical protein. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx. 90 kDa band observed in Human Brain (Cerebellum) lysates (calculated MW of 99.2 kDa according to NP_001070711.1). Recommended concentration |
| Immunohistochemistry | In paraffin embedded Human Cortex shows cytoplasm staining of neuronal cells. Recommended concentration, 5-10µg/ml. |
| Reference(s) | Earnshaw BA, Bressloff PC. Biophysical model of AMPA receptor trafficking and its regulation during long-term potentiation/long-term depression. J Neurosci. 2006 Nov 22;26(47) |