Goat anti-LASS1 Antibody |
| Principle Name | LASS1 |
| Official Symbol | LASS1 |
| All Name and Symbols | LAG1; CerS1; LASS1; LAG1 homolog, ceramide synthase 1; MGC90349; UOG1; LAG1 longevity assurance homolog 1; longevity assurance (LAG1, S. cerevisiae) homolog 1; longevity assurance gene 1; upstream of GDF1 |
| Accession Number(s) | NP_067090.1; NP_937850.1 |
| Peptide Sequence | KDLREYDTAEAQ, is from internal region |
| Human Gene ID(s) | 10715 |
| Non-Human GeneID(s) | 93898 (mouse); 306351 (rat); |
| Important Comments | This antibody is expected to recognize both reported isoforms (NP_067090.1and NP_937850.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | Approx. 37 kDa band observed in Human Brain (Cerebellum, Cerebral Cortex and Frontal Cortex) lysates (calculated MW of 38.0 kDa according to NP_937850.1). Recommended concentration |
| Reference(s) | Venkataraman K, Riebeling C, Bodennec J, Riezman H, Allegood JC, Sullards MC, Merrill AH Jr, Futerman AH. Upstream of growth and differentiation factor 1 (uog1), a mammalian homolog of the yeast longevity assurance gene 1 (LAG1), regulates N-stearoyl-sphinganine (C18-(dihydro)ceramide) synthesis in a fumonisin B1-independent manner in mammalian cells. J Biol Chem. 2002 Sep 20;277(38) |
