Goat anti-PCSK6 Antibody |
| Principle Name | PCSK6 |
| Official Symbol | PCSK6 |
| All Name and Symbols | PCSK6; proprotein convertase subtilisin/kexin type 6; PACE4; SPC4; paired basic amino acid cleaving system 4; subtilisin-like proprotein convertase 4; subtilisin-like protease; subtilisin/kexin-like protease PACE4 |
| Accession Number(s) | NP_002561.1; NP_612192.1; NP_612195.1; NP_612197.1; NP_612196.1; NP_612198.2; NP_612193.1; NP_612194.1 |
| Peptide Sequence | YHTFSAHQSRSR, is from internal region |
| Human Gene ID(s) | 5046 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognize reported isoforms a, b, g and h (NP_002561.1; NP_612192.1; NP_612193.1; NP_612194.1 resp.). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Preliminary experiments gave an approx 40-45 kDa band in Human Brain (Cerebellum) lysates after 0.2 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calcul |
| Immunohistochemistry | In paraffin embedded Human Skeletal Muscle shows striated staining in alternating fibres. Recommended concentration, 5-10µg/ml. |
| Reference(s) | Jin W, Wang X, Millar JS, Quertermous T, Rothblat GH, Glick JM, Rader DJ. Hepatic proprotein convertases modulate HDL metabolism. Cell Metab. 2007 Aug;6(2) |
