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Goat anti-MARCH10 Antibody
Item#:
dAP-1575
Size Price Quantity
50.0 ug $310.00
Goat anti-MARCH10 Antibody
Principle Name MARCH10
Official Symbol MARCH10
All Name and Symbols MARCH10; membrane-associated ring finger (C3HC4) 10 ; FLJ35757; MARCH-X; RNF190; ring finger protein 190
Accession Number(s) NP_001094345.1; NP_689811.2
Peptide Sequence SRNYPQPRTEE, is from internal region
Human Gene ID(s) 162333
Non-Human GeneID(s) 303596 (rat);
Important Comments This antibody is expected to recognise both reported isoforms (NP_001094345.1; NP_689811.2), which are identical.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB, IHC
Tested Species Human, Rat
Peptide ELISA antibody detection limit dilution 1 to 8000.
Western Blot Approx. 90 kDa band observed in Rat Testis lysates (calculated MW of 90.5 kDa according to Human NP_689811.2 and of 87.9 kDa according to Mouse NP_766156.2). Recommended concentration
Immunohistochemistry Paraffin embedded Human Testis. Recommended concentration
Reference(s) Morokuma Y, Nakamura N, Kato A, Notoya M, Yamamoto Y, Sakai Y, Fukuda H. Yamashina S, Hirata Y, Hirose S. MARCH-XI, a novel transmembrane ubiquitin ligase implicated in ubiquitin-dependent protein sorting in developing spermatids. J Biol Chem. 2007 Aug 24;282(34)

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