Goat anti-ATG5 Antibody |
| Principle Name | ATG5 |
| Official Symbol | ATG5 |
| All Name and Symbols | ATG5; ATG5 autophagy related 5 homolog (S. cerevisiae); APG5; APG5L; ASP; hAPG5; APG5 (autophagy 5, S. cerevisiae)-like; APG5 autophagy 5-like; ATG5 autophagy related 5-like; OTTHUMP00000040507; apoptosis specific protein |
| Accession Number(s) | NP_004840.1 |
| Peptide Sequence | SQVINEMQKKDHKQ, is from internal region |
| Human Gene ID(s) | 9474 |
| Non-Human GeneID(s) | 11793 (mouse); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA |
| Tested Species | |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | Preliminary experiments gave an approx. 38 kDa band in NIH3T3 lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 32.4 kDa a |
| Reference(s) | Lee YR, Lei HY, Liu MT, Wang JR, Chen SH, Jiang-Shieh YF, Lin YS, Yeh TM, Liu CC, Liu HS. Autophagic machinery activated by dengue virus enhances virus replication. Virology. 2008 May 10;374(2) |
