Goat anti-PRKAG3 Antibody

Item#:

dAP-1573

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-PRKAG3 Antibody
Principle Name	PRKAG3
Official Symbol	PRKAG3
All Name and Symbols	PRKAG3 ; protein kinase, AMP-activated, gamma 3 non-catalytic subunit ; AMPKG3; 5'-AMP-activated protein kinase, gamma-3 subunit; AMP-activated protein kinase, non-catalytic gamma-3 subunit; AMPK gamma-3 chain
Accession Number(s)	NP_059127.2
Peptide Sequence	KLGWDDELRKPGAQ, is from internal region
Human Gene ID(s)	53632
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 8000.
Western Blot	Preliminary experiments in Human Heart, Skeletal Muscle and Pancreas lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results b
Reference(s)	Costford SR, Kavaslar N, Ahituv N, Chaudhry SN, Schackwitz WS, Dent R, Pennacchio LA, McPherson R, Harper ME. Gain-of-function R225W mutation in human AMPKgamma3 causing increased glycogen and decreased triglyceride in skeletal muscle. PLoS ONE. 2007 Sep 19;2(9)