Goat anti-SPHK1 Antibody |
| Principle Name | SPHK1 |
| Official Symbol | SPHK1 |
| All Name and Symbols | SPHK1; sphingosine kinase 1; SPHK; SK1; SKase |
| Accession Number(s) | NP_068807.2; NP_892010.2; NP_001136073.1 |
| Peptide Sequence | DVDLESEKYRRLGE, is from internal region |
| Human Gene ID(s) | 8877 |
| Non-Human GeneID(s) | 20698 (mouse); 170897 (rat); |
| Important Comments | This antibody is expected to recognise all three reported isoforms (NP_068807.2; NP_892010.2; NP_001136073.1). Reported variants represent identical protein (NP_001136073.1; NP_001136074.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IP |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Approx. 40 kDa band observed in Human Peripheral Blood Mononucleocyte lysates (calculated MW of 43.9 kDa according to NP_068807.2). Recommended concentration |
| Reference(s) | Kase H, Hattori Y, Jojima T, Okayasu T, Tomizawa A, Suzuki K, Banba N, Monden T, Satoh H, Akimoto K, Kasai K. Globular adiponectin induces adhesion molecule expression through the sphingosine kinase pathway in vascular endothelial cells. Life Sci. 2007 Aug 23;81(11) |
