Goat anti-ERCC1 Antibody |
| Principle Name | ERCC1 |
| Official Symbol | ERCC1 |
| All Name and Symbols | ERCC1; excision repair cross-complementing rodent repair deficiency, complementation group 1 (includes overlapping antisense sequence); COFS4; UV20; excision repair cross-complementing 1; excision repair protein |
| Accession Number(s) | NP_973730.1; NP_001974.1 |
| Peptide Sequence | DPGKDKEGVPQPS, is from N Terminus |
| Human Gene ID(s) | 2067 |
| Non-Human GeneID(s) | |
| Important Comments | This antibody is expected to recognise both reported isoforms (NP_973730.1 and NP_001974.1). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | Approx 38kDa band observed in lysates of cell lines A431 and Kelly (calculated MW of 35.6kDa according to NP_973730.1). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. |
| Reference(s) | Zhang N, Liu X, Li L, Legerski R. Double-strand breaks induce homologous recombinational repair of interstrand cross-links via cooperation of MSH2, ERCC1-XPF, REV3, and the Fanconi anemia pathway. DNA Repair (Amst). 2007 Jul 30; [Epub ahead of print]..PMID |
