Goat anti-GNAT3 Antibody |
| Principle Name | GNAT3 |
| Official Symbol | GNAT3 |
| All Name and Symbols | GNAT3; guanine nucleotide binding protein, alpha transducing 3 ; tcag7.1053; GDCA; gustducin alpha-3 |
| Accession Number(s) | NP_001095856.1 |
| Peptide Sequence | KNQFLDLNLKKEDKE, is from internal region |
| Human Gene ID(s) | 346562 |
| Non-Human GeneID(s) | 242851 (mouse); 286924 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Preliminary experiments gave an approx 27kDa band in Human Colon and Testis lysates and in cell lines NIH 3T3, A431 and Kelly after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for |
| Immunohistochemistry | In paraffin embedded Human Small Intestine shows staining of endocrine cells amongst the enterocytes. Recommended concentration |
| Reference(s) | Jang HJ, Kokrashvili Z, Theodorakis MJ, Carlson OD, Kim BJ, Zhou J, Kim HH, Xu X, Chan SL, Juhaszova M, Bernier M, Mosinger B, Margolskee RF, Egan JM. Gut-expressed gustducin and taste receptors regulate secretion of glucagon-like peptide-1. Proc Natl Acad Sci U S A. 2007 Sep 18;104(38) |
