Goat anti-IA2 Antibody |
| Principle Name | IA2 |
| Official Symbol | PTPRN |
| All Name and Symbols | IA-2 ; protein tyrosine phosphatase, receptor type, N ; FLJ16131 ; IA-2/PTP; IA2 ; ICA512 ; R-PTP-N; islet antigen 2 ; islet cell antigen 512 ; islet cell autoantigen 3 ; protein tyrosine phosphatase-like N |
| Accession Number(s) | NP_002837.1 |
| Peptide Sequence | QGEGNIKKNRHPD, is from internal region |
| Human Gene ID(s) | 5798 |
| Non-Human GeneID(s) | 19275 (mouse); 116660 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Mouse |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Approx 110kDa band observed in lysates of cell line NIH 3T3 (calculated MW of 106kDa according to Human NP_002837.1 and Mouse NP_033011.2). Recommended concentration |
| Reference(s) | Buzzetti R, Di Pietro S, Giaccari A, Petrone A, Locatelli M, Suraci C, Capizzi M, Arpi ML, Bazzigaluppi E, Dotta F, Bosi E; Non Insulin Requiring Autoimmune Diabetes Study Group. High titer of autoantibodies to GAD identifies a specific phenotype of adult-onset autoimmune diabetes. Diabetes Care. 2007 Apr;30(4) |
