Goat anti-Mcp10 / Mcp9 Antibody

Item#:

dAP-1552

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Mcp10 / Mcp9 Antibody
Principle Name	Mcp10 / Mcp9
Official Symbol	Cma2
All Name and Symbols	Cma2; Chymase 2, mast cell; Mast cell protease 10; Mcp10;
Accession Number(s)	NP_001019885.1; NP_034912.3
Peptide Sequence	CKIFKHYKDSLQ, is from intenal region
Human Gene ID(s)
Non-Human GeneID(s)	17232 (mouse);
Important Comments	Mcp 10 and Mcp 9 (GeneID 545055) are almost identical in their protein sequence. No cross-reactivity expected with the similar Mcp4.
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 2000.
Western Blot	Preliminary experiments gave an approx 49kDa band in Human Skeletal Muscle lysates after 0.02µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated si
Reference(s)	Lee M, Calabresi L, Chiesa G, Franceschini G, Kovanen PT. Mast cell chymase degrades apoE and apoA-II in apoA-I-knockout mouse plasma and reduces its ability to promote cellular cholesterol efflux. Arterioscler Thromb Vasc Biol. 2002 Sep 1;22(9)