Goat anti-DAGLA Antibody |
| Principle Name | DAGLA |
| Official Symbol | DAGLA |
| All Name and Symbols | DAGLA; diacylglycerol lipase, alpha; C11orf11; DAGL(ALPHA); DAGLALPHA; NSDDR; diacylglycerol lipase alpha; neural stem cell-derived dendrite regulator; KIAA0659 |
| Accession Number(s) | NP_006124.1 |
| Peptide Sequence | PAKQDELVISAR, is from C Terminus |
| Human Gene ID(s) | 747 |
| Non-Human GeneID(s) | 269060 (mouse); 309207 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 128000. |
| Western Blot | Approx 125kDa band observed in Human Liver lysates (calculated MW of 115kDa according to NP_006124.1). Recommended concentration |
| Immunohistochemistry | Paraffin embedded Human Breast, Pancreas and Spleen. Recommended concentration |
| Reference(s) | Bisogno T, Howell F, Williams G, Minassi A, Cascio MG, Ligresti A, Matias I, Schiano-Moriello A, Paul P, Williams EJ, Gangadharan U, Hobbs C, Di Marzo V, Doherty P. Cloning of the first sn1-DAG lipases points to the spatial and temporal regulation of endocannabinoid signaling in the brain. J Cell Biol. 2003 Nov 10;163(3) |
