Goat anti-Dachshund homolog 1 Antibody

Item#:

dAP-1550

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Dachshund homolog 1 Antibody
Principle Name	Dachshund homolog 1
Official Symbol	DACH1
All Name and Symbols	OTTHUMP00000040839; dachshund homolog 1 (Drosophila) ; DACH1; RP11-512J14.1; DACH; FLJ10138; dachshund homolog 1
Accession Number(s)	NP_542937.1; NP_542938.1; NP_004383.2
Peptide Sequence	SPVENTPQNNECK, is from internal region
Human Gene ID(s)	1602
Non-Human GeneID(s)	13134 (mouse);
Important Comments	This antibody is expected to recognise all three reported isoforms(NP_542937.1; NP_542938.1; NP_004383.2).
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 16000.
Western Blot	Approx 75 and 52kDa bands observed in Human Kidney lysates (calculated MW of 73.0kDa according to NP_542937.1 and of 51.9kDa according to NP_004383.2). Recommended concentration
Reference(s)	Wu K, Yang Y, Wang C, Davoli MA, D'Amico M, Li A, Cveklova K, Kozmik Z, Lisanti MP, Russell RG, Cvekl A, Pestell RG. DACH1 inhibits transforming growth factor-beta signaling through binding Smad4. J Biol Chem. 2003 Dec 19;278(51)