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Goat anti-CHRNA4 (aa29-43) Antibody
Item#:
dAP-1549
Size Price Quantity
50.0 ug $310.00
Goat anti-CHRNA4 (aa29-43) Antibody
Principle Name CHRNA4 (aa29-43)
Official Symbol CHRNA4
All Name and Symbols CHRNA4; cholinergic receptor, nicotinic, alpha 4; BFNC; EBN; EBN1; NACRA4;cholinergic receptor, nicotinic, alpha 4 subunit; cholinergic receptor, nicotinic, alpha polypeptide 4; neuronal acetylcholine receptor alpha 4 subunit; neuronal nicotinic acetylcho
Accession Number(s) NP_000735.1
Peptide Sequence HVETRAHAEERLLKK, is from internal region
Human Gene ID(s) 1137
Non-Human GeneID(s) 11438 (mouse); 25590 (rat);
Important Comments This antibody is expected NOT to cross-react with the similar alpha 2 subunit.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, WB
Tested Species Human, Rat
Peptide ELISA antibody detection limit dilution 1 to 64000.
Western Blot Approx 70kDa band observed in Rat Brain lysates (calculated MW of 70.0kDa according to Human NP_000735.1and of 70.2kDa according to Rat NP_077330.1). Recommended concentration
Reference(s) Schwarz J, Schwarz SC, Dorigo O, Stützer A, Wegner F, Labarca C, Deshpande P, Gil JS, Berk AJ, Lester HA. Enhanced expression of hypersensitive alpha4* nAChR in adult mice increases the loss of midbrain dopaminergic neurons. FASEB J. 2006 May;20(7)

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