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Goat anti-LASS5+6 / CerS5+6 Antibody
Item#:
dAP-1547
Size Price Quantity
50.0 ug $310.00
Goat anti-LASS5+6 / CerS5+6 Antibody
Principle Name LASS5+6 / CerS5+6
Official Symbol LASS5
All Name and Symbols LASS5; LAG1 homolog, ceramide synthase 5; CerS5; FLJ25304; MGC45411; Trh4; LAG1 longevity assurance homolog 5
Accession Number(s) NP_671723.1; NP_982288.1
Peptide Sequence RGKVSKDDRSDVES, is from internal region
Human Gene ID(s) 91012; 253782
Non-Human GeneID(s) 71949 (mouse); 366984 (rat);
Important Comments This antibbody is expected to cross-react with the highly similar LASS6.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA
Tested Species
Peptide ELISA antibody detection limit dilution 1 to 4000.
Western Blot Preliminary experiments gave an approx. 60kDa band in lysates of HEK293, HeLa and HepG2 after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculate
Reference(s) Min J, Mesika A, Sivaguru M, Van Veldhoven PP, Alexander H, Futerman AH, Alexander S. (Dihydro)ceramide synthase 1 regulated sensitivity to cisplatin is associated with the activation of p38 mitogen-activated protein kinase and is abrogated by sphingosine kinase 1. Mol Cancer Res. 2007 Aug;5(8)

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