Goat anti-PU.1 Antibody |
| Principle Name | PU.1 |
| Official Symbol | SPI1 |
| All Name and Symbols | PU.1; SPI1; spleen focus forming virus (SFFV) proviral integration oncogene spi1 ; hCG_25181; SFPI1; SPI-1; SPI-A; 31 kDa transforming protein; SPI-1 proto-oncogene; hematopoietic transcription factor PU.1 |
| Accession Number(s) | NP_001074016.1; NP_003111.2 |
| Peptide Sequence | DLYQRQTHEYY, is from internal region |
| Human Gene ID(s) | 6688 |
| Non-Human GeneID(s) | 366126 (rat); |
| Important Comments | This antibody is expected to recognise both reported isoforms (NP_001074016.1; NP_003111.2). |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx 40kDa band observed in lysates of DAUDI and K562 (calculated MW of 31.2kDa according to NP_001074016.1 and of 31.1kDa according to NP_003111.2). The observed molecular weight corresponds to earlier findings in literature with differe |
| Immunohistochemistry | Paraffin embedded Human Spleen and Colon (lymphoid aggregate). Recommended concentration |
| Reference(s) | Uvarov P, Ludwig A, Markkanen M, Rivera C, Airaksinen MS. Upregulation of the neuron-specific K+/Cl- cotransporter expression by transcription factor early growth response 4. J Neurosci. 2006 Dec 27;26(52) |
