Goat anti-PITX3 Antibody |
Principle Name | PITX3 |
Official Symbol | PITX3 |
All Name and Symbols | PITX3; paired-like homeodomain 3; MGC12766; PTX3; homeobox protein PITX3 |
Accession Number(s) | NP_005020.1 |
Peptide Sequence | PEHGCKGQEHSD, is from internal region |
Human Gene ID(s) | 5309 |
Non-Human GeneID(s) | |
Important Comments | This antibody is not excpected to cross-react with PITX1 and PITX2. |
Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
Applications | Pep ELISA, WB |
Tested Species | Human |
Peptide ELISA | antibody detection limit dilution 1 to 1000. |
Western Blot | Approx 40kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 31.8kDa according to NP_005020.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (C |
Reference(s) | Kim J, Inoue K, Ishii J, Vanti WB, Voronov SV, Murchison E, Hannon G, Abeliovich A. A MicroRNA feedback circuit in midbrain dopamine neurons. Science. 2007 Aug 31;317(5842) |