Goat anti-MSH5 Antibody |
| Principle Name | MSH5 |
| Official Symbol | MSH5 |
| All Name and Symbols | MSH5; mutS homolog 5 (E. coli) ; XXbac-BCX40G17.5; DKFZp434C1615; G7N |
| Accession Number(s) | NP_079535.3 ; NP_751897.1; NP_002432.1 |
| Peptide Sequence | GDLHCEIRDQET, is from internal region |
| Human Gene ID(s) | 4439 |
| Non-Human GeneID(s) | 17687 (mouse); 294252 (rat); |
| Important Comments | This antibody is expected to recognise three reported isoforms ( NP_079535.3 ; NP_751897.1; NP_002432.1); Reported variants NP_751898.1 and NP_002432.1 represent identical protein. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | Preliminary experiments gave an approx 38kDa band in Human Testis, Tonsil and Thymus lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calc |
| Immunohistochemistry | In paraffin embedded Human Testis shows nuclear staining in spermatocytes. Recommended concentration, 2.5-3.8µg/ml. |
| Reference(s) | Neyton S, Lespinasse F, Lahaye F, Staccini P, Paquis-Flucklinger V, Santucci-Darmanin S. CRM1-dependent nuclear export and dimerization with hMSH5 contribute to the regulation of hMSH4 subcellular localization. Exp Cell Res. 2007 Oct 15;313(17) |
