Goat anti-USP28 Antibody |
| Principle Name | USP28 |
| Official Symbol | USP28 |
| All Name and Symbols | USP28; ubiquitin specific peptidase 28; KIAA1515; ubiquitin specific protease 28 |
| Accession Number(s) | NP_065937.1 |
| Peptide Sequence | SVELKHYIQEDN, is from internal region |
| Human Gene ID(s) | 57646 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | Preliminary experiments gave bands at approx 150kDa and 50kDa in Human Bone Marrow lysates after 0.2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calcu |
| Immunohistochemistry | In paraffin embedded Human Testis shows acrosomal staining in spermatids. Recommended concentration, 2.5-3.8µg/ml. |
| Reference(s) | Popov N, Herold S, Llamazares M, Schülein C, Eilers M. Fbw7 and Usp28 regulate myc protein stability in response to DNA damage. Cell Cycle. 2007 Aug;6(19) |
