Goat anti-ROBO2 Antibody |
| Principle Name | ROBO2 |
| Official Symbol | ROBO2 |
| All Name and Symbols | ROBO2; roundabout, axon guidance receptor, homolog 2 (Drosophila); KIAA1568; SAX3; roundabout, axon guidance receptor, homolog 2 |
| Accession Number(s) | NP_002933.1 |
| Peptide Sequence | NQSQRPRPTKKHK, is from internal region (near the C Terminus) |
| Human Gene ID(s) | 6092 |
| Non-Human GeneID(s) | 268902 (mouse); 84409 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA |
| Tested Species | |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Preliminary experiments gave an approx. 75kDa band in Human Brain (Amygdala) lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated s |
| Reference(s) | Fujisawa K, Wrana JL, Culotti JG. The slit receptor EVA-1 coactivates a SAX-3/Robo mediated guidance signal in C. elegans. Science. 2007 Sep 28;317(5846) |
