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Goat anti-Proenkephalin (mouse) Antibody
Item#:
dAP-1513
Size Price Quantity
50.0 ug $310.00
Goat anti-Proenkephalin (mouse) Antibody
Principle Name Proenkephalin (mouse)
Official Symbol PENK
All Name and Symbols proenkephalin
Accession Number(s) NP_001002927.1
Peptide Sequence YKDSSKQDESH, is from internal region
Human Gene ID(s)
Non-Human GeneID(s) 18619 (mouse); 29237 (rat);
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Mouse, Rat
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Preliminary experiments gave an approx 40kDa band in Rat Brain lysates after 0.2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.8kDa
Immunohistochemistry Frozen section of Rat Brain (Striatum) shows staining of dense enkephalinergic axon plexus. Frozen section of the Mouse Brain (Stria terminalis) shows staining of a dense enkephalinergic fibre network in the bed nucleus. Recommended
Reference(s) Schiltz CA, Bremer QZ, Landry CF, Kelley AE. Food-associated cues alter forebrain functional connectivity as assessed with immediate early gene and proenkephalin expression. BMC Biol. 2007 Apr 26;5

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