Goat anti-GCH1 Antibody |
| Principle Name | GCH1 |
| Official Symbol | GCH1 |
| All Name and Symbols | GCH1 ; GTP cyclohydrolase 1 (dopa-responsive dystonia) ; DYT5; GCH; GTP-CH-1; GTPCH1; GTP cyclohydrolase 1; guanosine 5'-triphosphate cyclohydrolase I |
| Accession Number(s) | NP_000152.1 ; NP_001019195.1 ; NP_001019241.1 ; NP_001019242.1 |
| Peptide Sequence | GKVHIGYLPNKQ, is from internal region |
| Human Gene ID(s) | 2643 |
| Non-Human GeneID(s) | 14528 (mouse); 29244 (rat); |
| Important Comments | This antibody is expected to recognize all four isoforms (NP_000152.1 ; NP_001019195.1 ; NP_001019241.1 ; NP_001019242.1) |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 16000. |
| Western Blot | Approx. 37kDa band observed in Human Tonsil lysates. Customer¿s experiments gave identical results in lysates of cell line HUVEC with antibodies raised against different parts of the same protein. (calculated MW of 27.9kDa according to NP_0 |
| Reference(s) | Tegeder I, Costigan M, Griffin RS, Abele A, Belfer I, Schmidt H, Ehnert C, Nejim J, Marian C, Scholz J, Wu T, Allchorne A, Diatchenko L, Binshtok AM, Goldman D, Adolph J, Sama S, Atlas SJ, Carlezon WA, Parsegian A, Lotsch J, Fillingim RB, Maixner W, Geiss GTP cyclohydrolase and tetrahydrobiopterin regulate pain sensitivity and persistence. Nat Med. 2006 Nov;12(11) |
