Goat anti-NRAS Antibody |
| Principle Name | NRAS |
| Official Symbol | NRAS |
| All Name and Symbols | NRAS ; neuroblastoma RAS viral (v-ras) oncogene homolog ; N-ras ; NRAS1; N-ras protein part 4 ; v-ras neuroblastoma RAS viral oncogene homolog |
| Accession Number(s) | NP_002515.1 |
| Peptide Sequence | RMKKLNSSDDGTQ, is from internal region |
| Human Gene ID(s) | 4893 |
| Non-Human GeneID(s) | 18176 (mouse); 24605 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB, IHC |
| Tested Species | Human, Rat |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Approx. 21kDa band observed in Human lysates of cell line A431 and of Rat Intestines (calculated MW of 21.2kDa according to NP_002515.1). Recommended concentration |
| Immunohistochemistry | In paraffin embedded Human Small Intestine shows staining of cytoplasm and membrane of enterocytes. Recommended concentration |
| Reference(s) | Thomas NE, Edmiston SN, Alexander A, Millikan RC, Groben PA, Hao H, Tolbert D, Berwick M, Busam K, Begg CB, Mattingly D, Ollila DW, Tse CK, Hummer A, Lee-Taylor J, Conway K. Number of nevi and early-life ambient UV exposure are associated with BRAF-mutant melanoma. Cancer Epidemiol Biomarkers Prev. 2007 May;16(5) |
