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Goat anti-Frizzled 2 Antibody
Item#:
dAP-1504
Size Price Quantity
50.0 ug $310.00
Goat anti-Frizzled 2 Antibody
Principle Name Frizzled 2
Official Symbol FZD2
All Name and Symbols FZD2 ; frizzled homolog 2 (Drosophila) ; frizzled 2
Accession Number(s) NP_001457.1
Peptide Sequence RYATLEHPFHCPR, is from internal region
Human Gene ID(s) 2535
Non-Human GeneID(s) 57265 (mouse); 64512 (rat);
Important Comments
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 64000.
Western Blot Preliminary experiments gave an approx. 23kDa band in Human Colon, Heart and Ovary lysates after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calcul
Immunohistochemistry In paraffin embedded Human Thyroid Gland shows cytoplasm and membranous staining in epithelial cells of the follicles. Recommended concentration
Reference(s) Oishi I, Kawakami Y, Raya A, Callol-Massot C, Izpisua Belmonte JC. Regulation of primary cilia formation and left-right patterning in zebrafish by a noncanonical Wnt signaling mediator, duboraya. Nat Genet. 2006 Nov;38(11)

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