Goat anti-OTUB1 Antibody |
| Principle Name | OTUB1 |
| Official Symbol | OTUB1 |
| All Name and Symbols | OTUB1; OTU domain, ubiquitin aldehyde binding 1; FLJ20113; FLJ40710; HSPC263; MGC111158; MGC4584; OTB1; OTU1; OTU-domain Ubal-binding 1; otubain 1; ubiquitin-specific protease otubain 1 |
| Accession Number(s) | NP_060140.2 |
| Peptide Sequence | YKEYAEDDNIYQQK, is from internal region |
| Human Gene ID(s) | 55611 |
| Non-Human GeneID(s) | 107260 (mouse); 293705 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, WB |
| Tested Species | Mouse |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | Approx. 30kDa band observed in Mouse Brain lysates (calculated MW of 31.3kDa according to Human NP_060140.2 and Mouse NP_598911.1). Recommended concentration |
| Reference(s) | Juris SJ, Shah K, Shokat K, Dixon JE, Vacratsis PO. Identification of otubain 1 as a novel substrate for the Yersinia protein kinase
using chemical genetics and mass spectrometry. FEBS Lett. 2006 Jan 9;580(1) |