Goat anti-Apolipoprotein F Antibody

Item#:

dAP-1485

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Apolipoprotein F Antibody
Principle Name	Apolipoprotein F
Official Symbol	APOF
All Name and Symbols	Apolipoprotein F; APOF; Apo-F; DKFZp781G18150; MGC22520
Accession Number(s)	NP_001629.1
Peptide Sequence	KSYDLDPGAGSLE, is from C Terminus
Human Gene ID(s)	319
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA, WB, IHC
Tested Species	Human
Peptide ELISA	antibody detection limit dilution 1 to 32000.
Western Blot	Approx. 35kDa band observed in Human Liver lysates (calculated MW of 35.4kDa according to NP_001629.1). Recommended concentration
Immunohistochemistry	In paraffin embedded Human Liver shows staniing of droplets in the cytoplasm of hepatocytes. Recommended concentration, 2.5-3.8µg/ml.
Reference(s)	Wang X, Driscoll DM, Morton RE. Molecular cloning and expression of lipid transfer inhibitor protein reveals its identity with apolipoprotein F. J Biol Chem. 1999 Jan 15;274(3)