Goat anti-DUBA Antibody |
| Principle Name | DUBA |
| Official Symbol | OTUD5 |
| All Name and Symbols | OTUD5; OTU domain containing 5; DKFZp761A052; DUBA; MGC104871; - |
| Accession Number(s) | NP_060072.1 |
| Peptide Sequence | RQVRGPSQPRKAS, is from internal region |
| Human Gene ID(s) | 55593 |
| Non-Human GeneID(s) | 54644 (mouse); 363452 (rat); |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA |
| Tested Species | |
| Peptide ELISA | antibody detection limit dilution 1 to 64000. |
| Western Blot | Preliminary experiments gave an approx 80kDa band in lysates of cell lines Jurkat and MOLT4 after 0.2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calcu |
| Reference(s) | Kayagaki N, Phung Q, Chan S, Chaudhari R, Quan C, O'Rourke KM, Eby M, Pietras E, Cheng G, Bazan JF, Zhang Z, Arnott D, Dixit VM. DUBA |
