Goat anti-Frizzled 3 Antibody

Item#:

dAP-1463

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-Frizzled 3 Antibody
Principle Name	Frizzled 3
Official Symbol	FZD3
All Name and Symbols	FZD3 ; frizzled homolog 3 (Drosophila) ; Fz-3; hFz3; frizzled 3
Accession Number(s)	NP_059108.1
Peptide Sequence	DDQRSKAGSIHSK, is from internal region
Human Gene ID(s)	7976
Non-Human GeneID(s)	14365 (mouse);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 2000.
Western Blot	Preliminary experiments in Human Brain (Frontal Cortex), Mouse Brain and Rat Brain lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have
Reference(s)	You J, Nguyen AV, Albers CG, Lin F, Holcombe RF. Wnt Pathway-Related Gene Expression in Inflammatory Bowel Disease. Dig Dis Sci. 2007 Oct 16; [Epub ahead of print].PMID