Goat anti-NDFIP1 Antibody

Item#:

dAP-1457

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-NDFIP1 Antibody
Principle Name	NDFIP1
Official Symbol	NDFIP1
All Name and Symbols	NDFIP1; Nedd4 family interacting protein 1; MGC10924; N4WBP5 ; Nedd4 WW domain-binding protein 5
Accession Number(s)	NP_085048.1
Peptide Sequence	YFDYKDESGFPKP, is from internal region
Human Gene ID(s)	80762
Non-Human GeneID(s)	65113 (mouse); 291609 (rat);
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 64000.
Western Blot	Preliminary experiments in Human Brain (Frontal Cortex) and Mouse and Rat Brain lysates gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have an
Reference(s)	Oliver PM, Cao X, Worthen GS, Shi P, Briones N, MacLeod M, White J, Kirby P, Kappler J, Marrack P, Yang B. Ndfip1 protein promotes the function of itch ubiquitin ligase to prevent T cell activation and T helper 2 cell-mediated inflammation. Immunity. 2006 Dec;25(6)