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Goat anti-Sterol carrier protein 2 Antibody
Item#:
dAP-1444
Size Price Quantity
50.0 ug $310.00
Goat anti-Sterol carrier protein 2 Antibody
Principle Name Sterol carrier protein 2
Official Symbol SCP2
All Name and Symbols SCP2 ; sterol carrier protein 2; DKFZp686C12188; DKFZp686D11188; NLTP; NSL-TP; SCPX; nonspecific lipid-transfer protein; sterol carrier protein X
Accession Number(s) NP_001007099.1; NP_002970.2; NP_001180528.1; NP_001180529.1; NP_001180546.1
Peptide Sequence KNHKHSVNNPYSQ, is from internal region
Human Gene ID(s) 6342
Non-Human GeneID(s) 20280 (mouse); 25541 (rat);
Important Comments This antibody is expected to recognize isoform 1, 2, 6, 7 and 8 (NP_002970.2; NP_001007099.1; NP_001180529.1; NP_001180528.1; NP_001180546.1 respectively),
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 32000.
Western Blot Preliminary experiments gave an approx 50kDa band in Human Liver lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 59.0
Immunohistochemistry In paraffin embedded Human Liver shows speckled cytoplasm staining in hepatocytes. Recommended concentration, 3-6µg/ml.
Reference(s) Atshaves BP, Gallegos AM, McIntosh AL, Kier AB, Schroeder F. Sterol carrier protein-2 selectively alters lipid composition and cholesterol dynamics of caveolae/lipid raft vs nonraft domains in L-cell fibroblast plasma membranes. Biochemistry. 2003 Dec 16;42(49)

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