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Goat anti-GCC185 Antibody
Item#:
dAP-1437
Size Price Quantity
50.0 ug $310.00
Goat anti-GCC185 Antibody
Principle Name GCC185
Official Symbol GCC2
All Name and Symbols GCC2; GRIP and coiled-coil domain containing 2; GCC185; KIAA0336; CLL-associated antigen KW-11; CTCL tumor antigen se1-1; GRIP and coiled-coil domain-containing 2; GRIP coiled-coil protein GCC185, (Ran-binding protein 2-like 4)
Accession Number(s) NP_055450.1; NP_852118.1
Peptide Sequence QKLTNKNNKIED, is from internal region
Human Gene ID(s) 9648
Non-Human GeneID(s)
Important Comments This antibody is expected to recognise both reported isoforms (NP_055450.1and NP_852118.1).
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Pep ELISA, IHC
Tested Species Human
Peptide ELISA antibody detection limit dilution 1 to 16000.
Western Blot Preliminary experiments gave an approx 60kDa band in Human Lymph Node and Testis lysates after 0.03µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calcula
Immunohistochemistry In paraffin embedded Human Spleen shows select staning of splenocytes. Recommended concentration, 3-6µg/ml.
Reference(s) Efimov A, Kharitonov A, Efimova N, Loncarek J, Miller PM, Andreyeva N, Gleeson P, Galjart N, Maia AR, McLeod IX, Yates JR 3rd, Maiato H, Khodjakov A, Akhmanova A, Kaverina I. Asymmetric CLASP-dependent nucleation of noncentrosomal microtubules at the trans-Golgi network. Dev Cell. 2007 Jun;12(6)

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