Goat anti-FZD9 Antibody |
| Principle Name | FZD9 |
| Official Symbol | FZD9 |
| All Name and Symbols | FZD9 ; frizzled homolog 9 (Drosophila) ; FZD3; frizzled 9 |
| Accession Number(s) | NP_003499.1 |
| Peptide Sequence | TKTDPSLENPTH, is from C Terminus |
| Human Gene ID(s) | 8326 |
| Non-Human GeneID(s) | |
| Important Comments | |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Supplied As | lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing. |
| Applications | Pep ELISA, IHC |
| Tested Species | Human |
| Peptide ELISA | antibody detection limit dilution 1 to 32000. |
| Western Blot | Preliminary experiments in Kidney lysates from Human, Mouse and Rat gave no specific signal but low background (at antibody concentration up to 1µg/ml). We would appreciate any feedback from people in the field - have any results been repo |
| Immunohistochemistry | In paraffin embedded Human Testis shows acrosomal staining of spermatids. Recommended concentration, 5-10µg/ml. |
| Reference(s) | Winn RA, Van Scoyk M, Hammond M, Rodriguez K, Crossno JT Jr, Heasley LE, Nemenoff RA. Antitumorigenic effect of Wnt 7a and Fzd 9 in non-small cell lung cancer cells is mediated through ERK-5-dependent activation of peroxisome proliferator-activated receptor gamma. J Biol Chem. 2006 Sep 15;281(37) |
