Goat anti-ERN2 / IRE1b Antibody

Item#:

dAP-1421

Size	Price	Quantity
50.0 ug	$310.00

Goat anti-ERN2 / IRE1b Antibody
Principle Name	ERN2 / IRE1b
Official Symbol	ERN2
All Name and Symbols	ERN2; endoplasmic reticulum to nucleus signaling 2 ; IRE1b; ER to nucleus signalling 2; IRE1 beta; IRE1, S. cerevisiae, homolog of; endoplasmic reticulum to nucleus signalling 2; inositol-requiring 1 beta
Accession Number(s)	NP_150296.3
Peptide Sequence	QKRLQSPSKQAQPLD, is from internal region
Human Gene ID(s)	10595
Non-Human GeneID(s)
Important Comments
Purification	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied As	lyophilized powder of 50ug or 100ug IgG; Reconsititute IgG with 100ul or 200ul sterile DI Water and final product will be formulated as 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Applications	Pep ELISA
Tested Species
Peptide ELISA	antibody detection limit dilution 1 to 4000.
Western Blot	Preliminary experiments gave an approx 37kDa band in Human Duodenum, Pancreas and Placenta lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given th
Reference(s)	Brewer JW, Diehl JA. PERK mediates cell-cycle exit during the mammalian unfolded protein response. Proc Natl Acad Sci U S A. 2000 Nov 7;97(23)